Vitrification Solutions
Vitrification Solutions Abstract Application: The use of freezing media in the glass freezing process is the safest method for freezing embryos and ovums before storing them in a liquid nitrogen tank
REF: RS-V001 3*1.5 ml
General information
Application: The use of freezing media in the glass freezing process is the safest method for freezing embryos and eggs before storing them in a liquid nitrogen tank.
Storage conditions and duration: Keep the product away from sunlight and at a temperature between 2-8°C. As long as the storage instructions are followed correctly, the product can be used until the expiration date indicated on the package.
Product description: ovum and embryo freezing environments
Quality assurance:
The melting media is sterilized by membrane filtration and has successfully passed the following tests:
endotoxin test (LAL)
Sterility test
Mouse embryo test (MEA)
instructions
The efficiency of the following protocol for using freezing environments in the glass freezing process is the safest method for freezing embryos and ovums before storage in a liquid nitrogen tank, and this protocol is presented only as an example:
(EMBRYO FREEZING PROTOCOL)
● The embryo is removed from the culture medium with a sterilized Pasteur pipette and it is inserted into the first drop (D1) of the V1 medium with a small amount of the vessel medium in which it was placed and kept in it for at least one minute.
Make sure to leave the embryo under the drop of V1 environment and start the timer immediately. Then again, with a Pasteur pipette, we remove the embryo from the first drop (D1) and transfer it to the second drop (D2) in the V1 environment and keep it in this environment for 6 to 9 minutes. After that, we transfer the embryo to the third drop (D3) from the V2 environment and keep it in that environment for one minute. After finishing the process, we quickly put the embryo on the freezing straw (cryotach) and put it into liquid nitrogen.
(OOCYTE FREEZING PROTOCOL)
● Remove the egg from the culture medium with a sterilized Pasteur pipette and insert it into the first drop (D1) of the V1 medium with a small amount of the vessel medium in which it was placed and keep it there for at least one and at most two minutes. we have
Make sure to leave the egg under the V1 droplet and start the timer immediately. Then again, with a Pasteur pipette, remove the egg from the first drop (D1) and transfer it to the second drop (D2) in the V1 environment and keep it in this drop for 13 to 14 minutes. It should be noted that the total time of egg freezing in V1 environment is considered to be 15 minutes. After that, we transfer the egg to the third drop (D3) from the V2 environment and keep it in that environment for one minute. After finishing the process, we quickly put the egg on the freezing straw (cryotach) and put it in liquid nitrogen.
●Note: For egg and embryo freezing, V1 and V2 mediums are removed from the refrigerator before starting work, and we use them after they reach the ambient temperature.
●Suggestion: Place up to three embryos or eggs on a cryotach to be frozen.
Warnings
1- To be used by trained and specialized people
2- If there is evidence of any foreign particles or microbial contamination, turbidity or sediment in the solution, avoid using it. If you see any damage in the sterile packaging of the solution, do not use it.
