Thawing Solutions
Thawing Solutions Abstract Application: The use of melting media in the glass freezing process is the safest method for thawing embryos and eggs after storage in a liquid nitrogen tank
REF: RS-T001 4*4 ml
General information
Application: The use of melting media in the glass freezing process is the safest method for thawing embryos and eggs after storage in a liquid nitrogen tank.
Storage conditions and duration: Keep the product away from sunlight and at a temperature between 2-8°C. As long as the storage instructions are followed correctly, the product can be used until the expiration date indicated on the package.
Product description: ovum and embryo melting media
Quality assurance:
The melting media is sterilized by membrane filtration and has successfully passed the following tests:
endotoxin test (LAL)
Sterility test
Mouse embryo test (MEA)
instructions
The effectiveness of the following protocol for using melting media in the glass freezing process is the safest method for thawing embryos and eggs after storage in a liquid nitrogen tank, and this protocol is presented only as an example:
●We remove the desired cryotach from the tank and put it in a cooling box in which we have already poured liquid nitrogen. Then place the said Cooling Box on the Work Station next to the microscope. We gently remove the cap of the cryotach under liquid nitrogen and lean the cryotach on the corner of the Cooling Box.
Note: Be careful not to remove the cap of the cryotach tube on which the embryo or egg is placed from the nitrogen.
Note: We lean the freezing straw (cryotach) on the Cooling Box in such a way that the embryo or egg is placed in the upper layer.
Note: About an hour before the start of work, we separate the environments as necessary.
●Place the T1 environment in the incubator at 37 degrees and put the T2 and T3 environments on the warm part of the Work Station until they reach the ambient temperature.
Pour the T1 medium in a dish and put it under the microscope. Quickly, by starting the timer, we remove the freeze straw (cryotach) from the nitrogen and enter the T1 environment and keep it in this environment for 1 minute.
●Then we remove the embryo or egg with a Pasteur pipette and transfer it to T2 environment and keep it in this environment for 3 minutes. After that, we remove the embryo or egg again and put them in the T3 environment and place them in this environment for 3 to 5 minutes. Then we remove the embryos or eggs from the T3 medium and wash them in the culture medium, then transfer them to the final drop and place them in the incubator.
Warnings
1- To be used by trained and specialized people
2- If there is evidence of any foreign particles or microbial contamination, turbidity or sediment in the solution, avoid using it.
If you see any damage in the sterile packaging of the solution, do not use it.
