REF: RS-HTF100 100 ml HTF culture medium brochure (download)
HTF culture medium production license (download)
HTF culture medium sterility test report (download)
In vitro fever test report of HTF culture medium (download)
Stimulation test report of HTF culture medium (download)
HTF culture medium sensitization test report (download)
HTF culture medium genotoxicity test report (download)
HTF culture medium cytotoxicity test report (download)
HTF culture medium package life test report (download) General information Application: collection of cumulus-ovum masses (COCs) and culture of human embryos from zygote stage to cleavage (third day)
Storage conditions and duration: Store in a refrigerator (2-8℃) and away from light. This product can be used up to 10 weeks after the production date (if the sterile packaging is not opened).
Considerations for waste disposal: It should be done according to the laboratory waste disposal instructions.
Ingredients: This product contains bicarbonate buffer, glucose, lactate and pyruvate needed to support the growth and development of human eggs and embryos in laboratory conditions.
instructions The efficiency of the following protocol for the collection of cumulus-ovum masses (COCs) and the culture of human embryos from the zygote stage to cleavage (day 3) has been shown, and this protocol is presented only as an example:
1- About 8-12 hours before use, the required amount of the culture medium should be mixed with the amount (volume-volume) of human serum albumin or HSA (Human Serum Albumin) with a concentration of 100 mg/ml to provide a protein supplement.
2- Volumes of 25-100 µl or more of the culture medium should be dripped in culture dishes and then completely covered by mineral oil.
3- Before placing the masses of COCs or embryos, the dish prepared in the previous step should be placed in the CO2 incubator for a sufficient period of time to ensure the pH and temperature balance. Depending on the conditions, this time may vary between 8 and 12 hours. If the culture medium and mineral oil have reached equilibrium before preparing the dishes in the incubator, the time required to balance the prepared dish will be shorter.
4- After transferring the masses of COCs to the drops of the culture medium, the dish should be kept in the CO2 incubator until denudation.
5- On the first day of embryo development, zygotes are placed in drops of culture medium and cultured in an incubator until the third day of development (4-8 cell stage).
Warnings 1- The user must read this manual completely and receive the necessary training before using the product.
2- This product cannot be used for injection into the body.
3- Do not re-sterilize the product.
4- Avoid using the product if you see the following:
- Presence of damage in sterile packaging
- The expiration date has passed
- Color change, turbidity or the presence of suspended particles or sediment.
5- This product contains the antibiotic gentamicin sulfate, so it should be used with caution in patients who are allergic to this antibiotic.
6- When using this product, in order to prevent contamination, aseptic methods should be used.
7- This product can be used safely up to 7 days after opening the sterile package.
Note: In most cases, the concentration of CO2 in the incubator at the rate of 5-7% will lead to the pH of the culture medium being in the accepted range of 7.2-7.4. However, fine-tuning the CO2 concentration to achieve an approximate pH of 7.3 (7.33 - 7.27) as the optimal pH depends on several factors, including the physical characteristics of the incubator and geographical altitude. As a result, it is recommended that each laboratory calculate the CO2 concentration required to achieve the optimal pH of 7.3 in the culture medium.
