REF: RS-SC30 30 ml
REF: RS-SC10 10 ml brochure of the single-stage embryo culture medium (download)
License for the construction of a single-stage embryo culture medium (download)
Report of sterility test of single-stage embryo culture medium (download)
The report of fever generation test using the LAL method of the single-stage embryo culture medium (download)
Genotoxicity test report of the single-stage embryo culture medium (download) General information Application: Human embryo culture from zygote to blastocyst stage and embryo transfer to the uterus
Storage conditions and duration: Store in a refrigerator (2-8˚C) and away from light. This product can be used up to 10 weeks after production (if the sterile packaging is not opened).
Considerations for waste disposal: It should be done according to the laboratory waste disposal instructions.
Ingredients: This product contains bicarbonate buffer, glucose, lactate, pyruvate and all 20 amino acids needed to support the growth and development of the human embryo in laboratory conditions.
instructions The efficiency of the following protocol for human embryo culture from the zygote to blastocyst stage as well as the transfer of the embryo to the uterus has been shown, and this protocol is presented only as an example:
1- About 8-12 hours before use, the required amount of the culture medium should be mixed with the amount (volume-volume) of human serum albumin or HSA (Human Serum Albumin) with a concentration of 100 mg/ml to provide a protein supplement.
2- Volumes of 100-25 µl or more of the culture medium should be dripped in culture dishes and then completely covered by mineral oil.
3- Before placing the embryos, the dish prepared in the previous step should be placed in the CO2 incubator for a sufficient period of time to ensure the pH and temperature balance. Depending on the conditions, this time may vary between 8 and 12 hours. If the culture medium and mineral oil have reached equilibrium before preparing the dishes in the incubator, the time required to balance the prepared dish will be shorter.
4- On the first day of embryo development, zygotes are placed in drops of culture medium and cultured in the incubator until the third day of development (4-8 cell stage).
5- Longer culture up to the blastocyst stage requires the transfer of cleavage stage embryos to a fresh culture medium in a new dish prepared in the above method.
6- To transfer the embryo to the uterus on the third day of development (cleavage stage) or on the fifth or sixth day (elastocyst stage), 20-30 µl of the balanced culture medium should be used.
7- Immediately before embryo transfer, the transfer counter should be washed with the culture medium.
Warnings 1- The user must read this manual completely and receive the necessary training before using the product.
2- This product cannot be used for injection into the body.
3- Do not re-sterilize the product.
4- Avoid using the product if you see the following:
- Presence of damage in sterile packaging
- The expiration date has passed
- Color change, turbidity or the presence of suspended particles or sediment.
5- This product contains the antibiotic gentamicin sulfate, so it should be used with caution in patients who are allergic to this antibiotic.
6- When using this product, in order to prevent contamination, aseptic methods should be used.
7- This product can be used safely up to 7 days after opening the sterile package.
Note: In most cases, the concentration of CO2 in the incubator at the rate of 5-7% will lead to the pH of the culture medium being in the accepted range of 7.2-7.4. However, fine-tuning the CO2 concentration to achieve an approximate pH of 7.3 (7.33 - 7.27) as the optimal pH depends on several factors, including the physical characteristics of the incubator and geographical altitude. As a result, it is recommended that each laboratory calculate the CO2 concentration required to achieve the optimal pH of 7.3 in the culture medium.
