REF: RS-SW100 100 ml
Brochure of sperm washing environment (download)
License to manufacture sperm washing medium (download)
Report of sterility test of sperm washing medium (download)
The report of in vitro fever generation test of sperm washing medium (download)
Test report of stimulation of sperm washing medium (download)
Report of sensitization test of sperm washing medium (download)
Genotoxicity test report of sperm washing medium (download)
report of cytotoxicity test of sperm washing medium (download)
Life test report of sperm washing medium packaging system (download) General information Application: Washing and preparing human sperm for use in ART processes
Storage conditions and duration: Store in a refrigerator at 2-8°C and away from light. This product can be used up to 6 months after production (if the sterile packaging is not opened).
Considerations for waste disposal: It should be done according to the laboratory waste disposal instructions.
Ingredients: This product contains bicarbonate buffer and HEPES, human serum albumin (HSA), glucose, lactate and pyruvate needed to meet the nutritional needs of sperm in laboratory conditions outside the CO2 incubator.
instructions The efficiency of the following protocol for sperm washing and preparation has been demonstrated, and this protocol is provided only as an example:
1- The required volume of the sperm washing medium is removed and placed in a CO2-free incubator for a sufficient period of time (1-2 hours) to ensure that the temperature is equalized. In case of using a CO2 incubator, make sure that the lid of the container containing the medium is completely closed in order to prevent pH drop.
2- Add 2ml of sperm washing medium to a tube containing 1ml of semen and place it in the incubator for 30-60 minutes. If using a CO2 incubator, make sure the lid of the container is completely closed in order to prevent pH drop. .
3- Aspiration and transferring the supernatant to a new tube and adding 5 ml of the sperm washing medium and pipetting.
4- Centrifuge washing twice for 10 minutes at 400-150 g.
5- Draining the supernatant and dissolving the sediment formed by adding 1 ml of the sperm washing medium.
Warnings 1- The user must read this manual completely and receive the necessary training before using the product.
2- This product cannot be used for injection into the body.
3- Do not re-sterilize the product.
4- Avoid using the product if you see the following:
- Presence of damage in sterile packaging
- The expiration date has passed
- Color change, turbidity or the presence of suspended particles or sediment.
5- This product contains the antibiotic gentamycin sulfate, so it should be used with caution in patients who are allergic to this antibiotic.
6- This product contains human serum albumin (HSA).
7- This medium contains small amounts of sodium bicarbonate buffer and therefore does not need to be exposed to CO2 to adjust the pH before or during use. Because this environment contains HEPES buffer, its exposure to CO2 causes toxicity.
8- When using this product, in order to prevent contamination, aseptic methods should be used.
9- This product can be used safely up to 7 days after opening the sterile packaging.
