REF: RS-ET 10 ml Embryo transfer environment brochure (download)
License for making embryo transfer environment (download)
Sterility test report of the embryo transfer medium (download)
Report of the in vitro fever test of embryo transfer environment (download)
report of febrile test by LAL method of embryo transfer medium (download)
Report of stimulation test of embryo transfer medium (download)
Embryo transfer medium sensitization test report (download)
Cytotoxicity test report of embryo transfer medium (download)
Test report on the longevity of the embryo transfer environment packaging system (download)
General information Application: Embryo transfer to human uterus
Storage conditions and duration: Store in a refrigerator (2-8℃) and away from light. This product can be used up to 10 weeks after the production date (if the sterile packaging is not opened).
Considerations for waste disposal: It should be done according to the laboratory waste disposal instructions.
Ingredients: This product contains bicarbonate buffer, human serum albumin (HSA), glucose, lactate, pyruvate and all amino acids and hyaluronan to support the growth and development of the human embryo and its implantation after transfer to the uterus.
instructions The efficiency of the following protocol for the transfer of human embryos to the uterus has been demonstrated, and this protocol is presented only as an example:
1- The required volume of the embryo transfer medium should be placed in the CO2 incubator for 4-8 hours to ensure the pH and temperature balance.
2- On the day of embryo transfer and 10 minutes before the procedure, the embryos are transferred to the embryo transfer environment in a dish and kept in the incubator until transfer. During this time, the lid of the dish should be closed to prevent surface evaporation.
3- Immediately before embryo transfer, the transfer counter should be washed with the culture medium.
4- To transfer the embryo to the uterus on the third day of development (cleavage stage), or on the fifth or sixth day (blastocyst stage), 20-30 µl of the balanced culture medium should be used.
Warnings 1- The user must read this manual completely and receive the necessary training before using the product.
2- This product cannot be used for injection into the body.
3- Do not re-sterilize the product.
4- Avoid using the product if you see the following:
- Presence of damage in sterile packaging
- The expiration date has passed
- Color change, turbidity or the presence of suspended particles or sediment.
5- This product contains the antibiotic gentamycin sulfate, so it should be used with caution in patients who are allergic to this antibiotic.
6- This product contains human serum albumin (HSA).
7- When using this product, in order to prevent contamination, aseptic methods should be used.
8- This product can be used safely up to 7 days after opening the sterile package.
Note: In most cases, the concentration of CO2 in the incubator at the rate of 5-7% will lead to the pH of the culture medium being in the accepted range of 7.2-7.4. However, fine-tuning the CO2 concentration to achieve an approximate pH of 7.3 (7.33 - 7.27) as the optimal pH depends on several factors, including the physical characteristics of the incubator and geographical altitude. As a result, it is recommended that each laboratory calculate the CO2 concentration required to achieve the optimal pH of 7.3 in the culture medium.
